PREPARATION OF ACETATE BUFFER
Aim
To prepare 0.1M acetate buffer of pH 4.
Procedure
Stock solution A : 0.2M solution of acetic acid
Stock solution B : 0.2M solution of sodium acetate
Mix 41 ml of A and 9ml of B and diluted to a
total of 100ml using distilled water. Measure pH using pH meter.
|
A (ml) |
B (ml) |
pH |
|
46.3 |
3.7 |
3.6 |
|
44 |
6 |
3.8 |
|
41 |
9 |
4 |
|
36.8 |
13.2 |
4.2 |
|
30.5 |
19.5 |
4.4 |
|
25.5 |
24.5 |
4.6 |
|
20 |
30 |
4.8 |
|
14.8 |
35.2 |
5 |
|
10.5 |
39.2 |
5.2 |
|
8.8 |
41.2 |
5.4 |
|
4.8 |
45.2 |
5.6
|
0.1 molar acetate buffer of pH 4 was prepared and confirmed by measuring pH using pH meter.
Aim
To prepare 0.1M carbonate
and bicarbonate buffer of pH 10.
Procedure
Stock solution A : 0.2M solution of anhydrous sodium carbonate
Stock solution B : 0.2M solution of sodium bicarbonate
Mix 27.5 ml of A
and 22.5ml of B and diluted to a total of 100ml using distilled water. Measure pH
using pH meter. Confirm the pH using pH meter.
|
A (ml) |
B (ml) |
pH |
|
4 |
46 |
9.2 |
|
9.5 |
40.5 |
9.4 |
|
16 |
34 |
9.6 |
|
22 |
28 |
9.8 |
|
27.5 |
22.5 |
10 |
|
33 |
17 |
10.2 |
|
38.5 |
11 |
10.4 |
|
42.5 |
7.5 |
10.6 |
0.1 molar
carbonate bicarbonate buffer of pH 10 was prepared and confirmed by measuring pH
using pH meter.
