Aim
To demonstrate the oligo dynamic action of heavy
metals like copper
Principle
The ability of small amount of heavy metals to
exert a lethal effect on bacteria is designated as oligo dynamic action. The
effectiveness of this small amounts of metal is probably due to the high
affinity of cellular proteins for the metallic ions. Although the concentration
of ion in solutions may be miniscule (few parts per million) cells die due to
the cumulative effects of ions within the cell.
Oligo dynamic action of certain other heavy metals
have been applied to water purification, ointment manufacturing and the
treatments of bandages and fabrics.
Materials
Required
Culture - Staphylococcus
aureus/ Escherichia coli/ Klebsiella/ Bacillus
Media - Nutrient agar
Equipments -
Petri plate, Bunsen burner, alcohol, sterile swabs, metallic disc of copper
Procedure
1. 1. Nutrient
agar was prepared and sterilized and poured into sterile petridishes.
2. 2. Sterile
cotton swab was moistened with broth culture of bacteria and was spread
uniformly over the surface of agar plate
3. 3. The
coin was surface sterilized using alcohol and placed at the center of the
nutrient agar plate with the help of a sterile forceps
4. \4. The
plates were incubated at 37oC for 24 hours
5. 5. The
diameter of the zone of inhibition/clearance around the copper coin was
measured.
Result
The diameter of the zone of clearance around the copper coin was 2.5 cm
Further Reading (optional, but recommended)
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