Aim: To prepare 0.1 M acetate buffer of pH 5.0
Procedure
Acetate buffer has a pH range of 3.6 to 5.6. To obtain a buffer of respective pH, mix A (acetic acid) and B (sodium acetate) in the proportions indicated below and adjust the final volume to 100 ml using distilled water The pH can be checked using a sensitive pH meter.
Stock solution A- 0.2 M solution of acetic acid
Stock solution B- 0.2 M solution of sodium acetate
Mixed 14.8 ml of A and 35.2 ml of B diluted to 100 ml using distilled water to obtain 0.1 M acetate buffer of pH 5.0
Solution A (ml) (Acetic acid) | Solution B (ml) (Sodium acetate) | pH |
46.3 | 3.7 | 3.6 |
44 | 6 | 3.8 |
41 | 9 | 4 |
36.8 | 13.2 | 4.2 |
30.5 | 19.5 | 4.4 |
25.5 | 24.5 | 4.6 |
20 | 30 | 4.8 |
14.8 | 35.2 | 5.0 |
10.5 | 39.2 | 5.2 |
8.8 | 41.2 | 5.4 |
4.8 | 45.2 | 5.6 |
Result
The buffer prepared is 0.1 M acetate buffer with pH 5. It is confirmed using a pH meter.
