Preparation of Acetate Buffer

 Aim: To prepare 0.1 M acetate buffer of pH 5.0 

Procedure

Acetate buffer has a pH range of 3.6 to 5.6. To obtain a buffer of respective pH, mix A (acetic acid) and B (sodium acetate) in the proportions indicated below and adjust the final volume to 100 ml using distilled water The pH can be checked using a sensitive pH meter.

Stock solution A- 0.2 M solution of acetic acid

Stock solution B- 0.2 M solution of sodium acetate

 Mixed 14.8 ml of A and 35.2 ml of B diluted to 100 ml using distilled water to obtain 0.1 M acetate buffer of pH 5.0

 

Solution A (ml) (Acetic acid)	Solution B (ml) (Sodium acetate)	pH
46.3	3.7	3.6
44	6	3.8
41	9	4
36.8	13.2	4.2
30.5	19.5	4.4
25.5	24.5	4.6
20	30	4.8
14.8	35.2	5.0
10.5	39.2	5.2
8.8	41.2	5.4
4.8	45.2	5.6

 Result

The buffer prepared is 0.1 M acetate buffer with pH 5. It is confirmed using a pH meter.