Preparation of Agar Slant and Agar Deep culture tubes

Aim

To prepare nutrient agar slant and deep culture tubes

Principle

Autoclaved agar medium poured into culture tubes and placed in slanting position results in slant after solidification. If the culture tubes are kept erect on solidification, it gives deep tubes.

Materials Required

Nutrient agar medium, sterilised test tubes, test tube stand, cotton plug, Bunsen burner

Procedure

1. 8-10 ml nutrient agar medium is poured into each test tube and cotton plugs put.

1. Tubes were transferred to test tube stand and autoclaved at 121^oC at 15 lbs pressure for 20 minutes.

2. One set of test tubes were kept in slanting position and second set of test tubes kept in test tube stand.

3. Medium was allowed to solidify.

Result

Agar slant and agar deep culture tubes were prepared.