Aim
To prepare nutrient broth for cultivation of bacteria.
Materials Required
Beef extract, Yeast extract, peptone, sodium chloride,
agar agar, distilled water, conical flask, weighing machine, measuring
cylinder, heater.
Procedure
1. Beef
extract, yeast extract, peptone and sodium chloride were weighed to the
required amount and added to a known volume of water.
2. They
were mixed well and dissolved.
3. pH
was adjusted to 6.8-7.2.
4. The
medium was poured to conical flask or tubes in the required amount.
5. Those
were plugged with cotton and autoclaved at 121oC at 15 lbs pressure
for 20 minutes.
6. The
medium was then cooled to hand bearable temperature and poured into sterile
test tubes as per requirement.
Result
A nutrient broth medium was prepared.
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